Phenotype Image Detail

Image

Caption

Thin filament length is increased in hearts of Hspb7^tm1.2Chen/Hspb7^tm1.2Chen embryos. (B) Immunofluorescence images (Upper) of two wild-type (WT) and Hspb7^tm1.2Chen/Hspb7^tm1.2Chen (KO) embryos from E10.5 heart sections. Z lines were stained using antibodies against alpha-actinin (Actn2; red), and actin (thin) filaments were stained using phalloidin (green). Quantification of WT vs. KO thin filament length (Lower). Two embryos and 12-20 sarcomeres per group. Error bars indicate mean SEM. Statistical significance was determined with two-tailed Student's t test. H, actin-free H zone; Z, Z lines. ****P < 0.0001. (Scale bar, 1 um.) (C) WT and KO E10.5 heart cryosections were stained with phalloidin (F actin) and antibodies against Actn2 and Tmod1. (Scale bar: 10 um.)

Copyright

This image is from Wu T, Proc Natl Acad Sci U S A 2017 Nov 7;114(45):11956-11961. Copyright 2017 National Academy of Sciences, U.S.A. J:256653

Associated Alleles

Symbol	Name
Hspb7^tm1.2Chen	heat shock protein family, member 7 (cardiovascular); targeted mutation 1.2, Ju Chen

Associated Genotypes

Allelic Composition	Genetic Background
Hspb7^tm1.2Chen/Hspb7^tm1.2Chen	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA