Phenotype Image Detail

Image

Caption

HSPB7 represses formation of abnormal actin bundles (AABs) independent of Tmod1. (A) Immunofluorescence images of wild-type (WT), Tg(Myh6-Tmod1)65Msus/Tg(Myh6-Tmod1)65Msus (Tmod1 Tg), Hspb7^tm1.2Chen/Hspb7^tm1.2Chen (HSPB7 KO), and Hspb7^tm1.2Chen/Hspb7^tm1.2Chen Tg(Myh6-Tmod1)65Msus/Tg(Myh6-Tmod1)65Msus (Tmod1 Tg/HSPB7 KO) E11.5 heart sections stained with antibodies against Tmod1 and alpha-actinin (Actn2). (Scale bar: 100 mm.) (B) Representative immunofluorescence images of WT, Tmod1 Tg, HSPB7 KO, and Tmod1 Tg/HSPB7 KO E10.5 heart cryosections stained with antibodies against Tmod1, Actn2, and phalloidin (F actin). Yellow arrowheads indicate AABs marked by Actn2 staining. Green arrowheads indicate thin filament pointed ends of WT sarcomeres marked by Tmod1 staining. Red arrowheads indicate thin filament pointed ends of Tmod1 Tg/HSPB7 KO sarcomeres marked by Tmod1 staining. (Scale bar: 10 mm.) (C) Representative immunofluorescence images (Left) and thin filament length measurements (Right) of WT, Tmod1 Tg, HSPB7 KO, and Tmod1 Tg/HSPB7 KO E10.5 embryos. Two embryos, 41-63 sarcomeres measured for each group. Error bars indicate mean SEM. WT, 0.819 0.0041 mm; Tmod1 Tg, 0.804 0.004 mm; HSPB7 KO, 0.851 0.0044 mm; HSPB7 KO/Tmod1 Tg, 0.849 0.0038 mm. Statistical significance was determined with one-way ANOVA. Not significant (N.S.; P = 0.9936). H, actin-free H zone; Z, Z lines. *P < 0.05 (P = 0.0275); ****P < 0.0001. (Scale bar, 1 um.)

Copyright

This image is from Wu T, Proc Natl Acad Sci U S A 2017 Nov 7;114(45):11956-11961. Copyright 2017 National Academy of Sciences, U.S.A. J:256653

Associated Alleles

Symbol	Name
Hspb7^tm1.2Chen	heat shock protein family, member 7 (cardiovascular); targeted mutation 1.2, Ju Chen
Tg(Myh6-Tmod1)65Msus	transgene insertion 65, Mark A Sussman

Associated Genotypes

Allelic Composition	Genetic Background
Hspb7^tm1.2Chen/Hspb7^tm1.2Chen Tg(Myh6-Tmod1)65Msus/Tg(Myh6-Tmod1)65Msus	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * FVB/N