Phenotype Image Detail

Image

Caption

Pax3-Cre-mediated Trpm7 disruption in kidney resulted in early defective nephrogenesis and a progressive kidney failure phenotype. (A) Haematoxylin and eosin (H&E) staining of kidney sections at E14.5, E18.5, and P2 from Trpm7^tm1Clph/Trpm7^tm1Clph Tg(Pax3-cre)1Joe/0 (ko) mice. Spherical renal vesicles were present in E14.5 ko embryonic kidney, but comma- and S-shaped bodies were clearly absent (compared with wild-type (wt) control; arrows). There were reduced glomeruli in ko embryonic kidney at E18.5 and dilated tubules in the renal cortex of ko kidney at P2. (B) High magnification images of dilated renal tubules in P2 ko kidney. (Left) Note even distribution of eosinophilic acellular material in the tubular lumen of ko (example circled). (Right) Note well-organized basement membrane in wt kidney (arrow), and damaged ones in ko kidney (diffuse purple in periodic acid-Schiff stain). (C) Proximal tubular cysts first appeared at P4 in ko kidney. The cysts were associated with proximal tubules (AQP1+) but not connecting tubules (D28K+), distal convoluted tubules (NCC+), or collecting ducts (AQP2+). Cysts are highlighted by white dots. Nuclei were stained by DAPI.

Copyright

This image is from Jin J, Proc Natl Acad Sci U S A 2012 Jan 31;109(5):E225-33. Copyright 2012 National Academy of Sciences, U.S.A. J:181963

Associated Alleles

Symbol	Name
Tg(Pax3-cre)1Joe	transgene insertion 1, Jonathan A Epstein
Trpm7^tm1Clph	transient receptor potential cation channel, subfamily M, member 7; targeted mutation 1, David E Clapham

Associated Genotypes

Allelic Composition	Genetic Background
Trpm7^tm1Clph/Trpm7^tm1Clph Tg(Pax3-cre)1Joe/0	involves: 129S4/SvJae * C57BL/6 * SJL