Phenotype Image Detail

Image

Caption

YY1 is required for melanocyte development in vivo. (A) Skin and hair pigmentation phenotype of Tg(Tyr-cre)1Lru/Y Yy1^tm2.1Yshi/Yy1^tm2.1Yshi (TyrCre, yy1^f/f) in the first (P4, P10) and second (P45) hair cycles. (B) H&E staining of hair follicles from skin sections of Tg(Tyr-cre)1Lru/Y Yy1^tm2.1Yshi/Yy1⁺ (TyrCre, yy1^f/+) and TyrCre, yy1^f/f mice in the first (P4) and second (P38) hair cycles. Arrows point to the hair follicles still containing pigment. (C,D) Immunofluorescence staining of DCT (red) in P4 hair follicles of TyrCre, yy1^f/+ (C) and TyrCre, yy1^f/f (D) mice. Skin sections were stained with goat anti-DCT primary antibody and donkey anti-goat Alexa 594 secondary antibody. Nuclei were counterstained with DAPI (blue). (E,F) Immunofluorescence staining of DCT (red) and YY1 (green) in P4 hair follicles of TyrCre, yy1^f/+ (E) and TyrCre, yy1^f/f (F) mice. Skin sections were double-stained with goat anti-DCT and rabbit anti-YY1 primary antibodies and then donkey anti-goat Alexa 594 and goat anti-rabbit Alexa 488 secondary antibodies. (G,H) Zoom-in view of the dashed box area in (F). DAPI stain is in blue in (H). (I,J) Immunofluorescence staining of DCT (red) in P38 hair follicles of TyrCre, yy1^f/+ (I) and TyrCre, yy1^f/f (J) mice. Nuclei were stained with DAPI (blue).

Copyright

This image is from Li J, PLoS Genet 2012 May;8(5):e1002688, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:185128

Associated Alleles

Symbol	Name
Tg(Tyr-cre)1Lru	transgene insertion 1, Lionel Larue
Yy1^tm2.1Yshi	YY1 transcription factor; targeted mutation 2.1, Yang Shi

Associated Genotypes

Allelic Composition	Genetic Background
Yy1^tm2.1Yshi/Yy1^tm2.1Yshi Tg(Tyr-cre)1Lru/Y	involves: 129S4/SvJae * C57BL/6 * DBA/2