Phenotype Image Detail

Image

Caption

Spermatogenic defects in Katnal2^{tm1a(EUCOMM)Wtsi}/Katnal2^{tm1a(EUCOMM)Wtsi} Tg(Stra8-icre)1Reb/0 mice. (a) Testis weight in Katnal2^{tm1c(EUCOMM)Wtsi}/Katnal2^{tm1c(EUCOMM)Wtsi} (Katnal2^FLOX/FLOX) (white triangles) and Katnal2^{tm1c(EUCOMM)Wtsi}/Katnal2^{tm1c(EUCOMM)Wtsi} Tg(Stra8-icre)1Reb/0 (Katnal2^GCKO/GCKO) (black triangles) mice (n = 3/group). (b) Daily sperm output (DSP) in the testes of FLOX/FLOX (white triangles; n = 3) and GCKO/GCKO (black triangles; n = 4) mice. (c) Total epididymal sperm content of FLOX/FLOX (white triangles) and GCKO/GCKO (black triangles) mice (n = 3/group). Total epididymal sperm content was reduced by 94.1% in GCKO/GCKO mice compared to FLOX/FLOX mice. In a-c lines represent mean SD, ** p<0.01 compared to FLOX/FLOX. Periodic acid Schiff's (PAS) stained testis sections from FLOX/FLOX and GCKO/GCKO mice (d-i and p-q). Low magnification view of seminiferous tubules in FLOX/FLOX (d) and GCKO/GCKO (e) mice. Multinucleated symplasts (arrowhead) were frequently observed in the GCKO/GCKO seminiferous epithelium. Elongating spermatids in FLOX/FLOX (f) versus GCKO/GCKO (g) mice. Abnormal nuclear morphology of spermatids (arrowheads) was frequently observed in GCKO/GCKO mice. Spermiation in FLOX/FLOX (h) versus GCKO/GCKO (i) mice. Retained elongated spermatids (arrowheads) were often observed in stage IX tubules of GCKO/GCKO mice but were rarely observed in FLOX/FLOX mice. Acetylated tubulin immunolabelling as a marker for sperm tail presence in FLOX/FLOX (j) and GCKO?GCKO (k) testis sections. Sperm tail content was markedly reduced in GCKO/GCKO (k) versus WT (j) mice. Scale bars in d-k = 10 um. Electron microscopy of stage VIII tubulobulbar complexes (asterisks) in FLOX/FLOX (l) versus GCKO/GCKO (m) mice. Tubulobulbar complex formation was normal in GCKO/GCKO mice. Scale bars in l-m= 2 um. Immunostaining of seminiferous tubules for the tubulobulbar complex marker ARP2 in FLOX/FLOX (n) versus GCKO/GCKO (o) mice further confirmed the presence of tubulobulbar complexes from GCKO/GCKO spermatids. Residual body (arrowheads) formation in FLOX/FLOX (p) versus GCKO/GCKO (q) mice. Residual body formation appeared normal in GCKO/GCKO mice. Scale bars in n-q = 10 um.

Copyright

This image is from Dunleavy JEM, PLoS Genet 2017 Nov;13(11):e1007078, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:250115

Associated Alleles

Symbol	Name
Katnal2^tm1c(EUCOMM)Wtsi	katanin p60 subunit A-like 2; targeted mutation 1c, Wellcome Trust Sanger Institute
Tg(Stra8-icre)1Reb	transgene insertion 1, Robert E Braun

Associated Genotypes

Allelic Composition	Genetic Background
Katnal2^tm1c(EUCOMM)Wtsi/Katnal2^tm1c(EUCOMM)Wtsi Tg(Stra8-icre)1Reb/0	involves: C57BL/6N * FVB/NJ