Phenotype Image Detail

Image

Caption

Disorganized bone matrix collagen arrangement in Col12a1^tm1Debi/Col12a1^tm1Debi (Col12a1-/-) mice. (A) Osteoblast morphology was analyzed in cross sections of the diaphysis from femurs of P30 wild-type (WT) and mutant mice with H&E staining. Osteoblasts in WT bone are localized on the surface of cortical bone (CB), where they are aligned and well organized in an epithelioid manner (arrows). In contrast, mutant osteoblasts (arrows) are poorly aligned and crowded in the periosteum (PO) with a disorganized arrangement associated with the surface of cortical bone. BM, bone marrow. (B) Collagen organization was analyzed in cross sections of the diaphysis from femurs of P14 WT and mutant mice. To analyze collagen organization, Sirius red staining with polarized light microscopy was performed. Collagen fibers (arrows) are arranged in parallel and are well organized in the WT cortical bone matrix, whereas birefringence is sparse in mutant mice, indicating smaller and/or less organized collagen fibers (asterisks) in the cortical bone. Bars, 50 um.

Copyright

This image is from Izu Y, J Cell Biol 2011 Jun 13;193(6):1115-30, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:174217

Associated Alleles

Symbol	Name
Col12a1^tm1Debi	collagen, type XII, alpha 1; targeted mutation 1, David E Birk

Associated Genotypes

Allelic Composition	Genetic Background
Col12a1^tm1Debi/Col12a1^tm1Debi	involves: 129 * C57BL/6