Phenotype Image Detail

Image

Caption

Altered cell-cell interaction/communication in Col12a1^tm1Debi/Col12a1^tm1Debi (Col12a1-/-) bone in vivo. Analysis of cross sections from the diaphysis of femurs in P14 wild-type (WT) and mutant mice using transmission EM. (A) WT osteoblasts (OB) attached to the surface of cortical bone (CB) are elongated and polarized relative to the bone matrix. In contrast, mutant osteoblasts (arrows) are disorganized and less polarized. PO, periosteum. (B and C) High magnification of adjacent osteoblasts in the periosteum. In WT bone, osteoblasts have numerous processes (double arrows) that connect osteoblast to osteoblast. Mutant osteoblasts have large intracellular spaces (asterisks) with fewer processes (double arrows). (D) Osteocyte within cortical bone matrix. Similar to the osteoblast phenotype, an osteocyte has processes (double arrows) that extend toward the cortical bone surface in WT bone connecting to other osteocytes or osteoblasts; however, mutant osteocytes have fewer and less developed processes (double arrows). Bars: (A) 5 um; (B) 1 um; (C) 500 nm; (D) 2 um.

Copyright

This image is from Izu Y, J Cell Biol 2011 Jun 13;193(6):1115-30, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:174217

Associated Alleles

Symbol	Name
Col12a1^tm1Debi	collagen, type XII, alpha 1; targeted mutation 1, David E Birk

Associated Genotypes

Allelic Composition	Genetic Background
Col12a1^tm1Debi/Col12a1^tm1Debi	involves: 129 * C57BL/6