Phenotype Image Detail

Image

Caption

Enhanced Wnt signaling and differentiation in crypts of Cdc25a^tm1Hpw/Cdc25a^tm1.1Hpw Cdc25b^tm1Pjd/Cdc25b^tm1Pjd Cdc25c^tm1Hpw/Cdc25c^tm1Hpw Gt(ROSA)26Sor^{tm1(cre/ERT)Brn}/Gt(ROSA)26Sor⁺ (TKO) mice. (A and B) Tissue sections of small intestines prepared from wild-type (WT) (A) and TKO (B) mice were stained with antibodies specific for Beta-catenin (green) and lysozyme (red). Nuclei were stained with DAPI (blue). Arrows indicate cells staining positive for nuclear Beta-catenin. (Scale bar: 10 um.) (C-G) Small intestines were dissected and processed for transmission electron microscopy. (D) An EM section of crypt from a WT mouse and higher magnification (C) showing a CBC cell between 2 mature Paneth cells, marked with an asterisk in (D). (E) EM sections of crypt from a TKO mouse. The inset in (E) is shown at higher magnification in (F). The arrow indicates an immature goblet cell with characteristic apical mucinogenic granules. In (F), a cell with stem-like morphology above the Paneth cell compartment is marked with an asterisk. (G) CBC cells differentiated along the Paneth cell lineage as marked by the appearance of small, electron-dense secretory granules. In (G), asterisks indicate the position of the intestinal lumen. [Scale bar: 10 um (D and E); 2 um (F and G).]

Copyright

This image is from Lee G, Proc Natl Acad Sci U S A 2009 Mar 24;106(12):4701-6. Copyright 2009 National Academy of Sciences, U.S.A. J:145768

Associated Alleles

Symbol	Name
Cdc25a^tm1.1Hpw	cell division cycle 25A; targeted mutation 1, Helen Piwnica-Worms
Cdc25a^tm1Hpw	cell division cycle 25A; targeted mutation 1, Helen Piwnica-Worms
Cdc25b^tm1Pjd	cell division cycle 25B; targeted mutation 1, Peter J Donovan
Cdc25c^tm1Hpw	cell division cycle 25C; targeted mutation 1, Helen Piwnica-Worms
Gt(ROSA)26Sor^tm1(cre/ERT)Brn	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Anton Berns

Associated Genotypes

Allelic Composition	Genetic Background
Cdc25a^tm1Hpw/Cdc25a^tm1.1Hpw Cdc25b^tm1Pjd/Cdc25b^tm1Pjd Cdc25c^tm1Hpw/Cdc25c^tm1Hpw Gt(ROSA)26Sor^tm1(cre/ERT)Brn/Gt(ROSA)26Sor^+	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6