Phenotype Image Detail

Image

Caption

Cdc25a^tm1.1Hpw/Cdc25a^tm1.1Hpw (Cdc25A^-/-) embryos undergo apoptosis during postimplantation development. (A) Whole uteri of pregnant females from heterozygous intercrosses were dissected at E7.5, then sectioned and stained with H&E (Top). The genotypes of each embryo are indicated. Adjacent sections were incubated with the terminal deoxyribonucleotide transferase and fluorescein-conjugated dUTP to label cells undergoing apoptosis (Bottom). The arrow depicts remnants of an embryo undergoing apoptosis. (Original magnification: 4X; scale bar: 200 um.) (B) Blastocysts were isolated by uterine flushing from heterozygous intercrosses at E3.5 and then cultured in vitro for 8 days. Photographs taken on days 0, 3, and 8 are shown. (Original magnification: 40X for day 0 and 10X for days 3 and 8). The photographs taken on day 3 were cropped and enlarged. (Scale bar: 100 um.) ICM, inner cell mass; TGC, trophoblast giant cell.

Copyright

This image is from Lee G, Proc Natl Acad Sci U S A 2009 Mar 24;106(12):4701-6. Copyright 2009 National Academy of Sciences, U.S.A. J:145768

Associated Alleles

Symbol	Name
Cdc25a^tm1.1Hpw	cell division cycle 25A; targeted mutation 1, Helen Piwnica-Worms

Associated Genotypes

Allelic Composition	Genetic Background
Cdc25a^tm1.1Hpw/Cdc25a^tm1.1Hpw	involves: 129X1/SvJ * C57BL/6