Phenotype Image Detail

Image

Caption

Ameloblast defects in Bcl11b^tm1.2Leid/Bcl11b^tm1.2Leid (Ctip2^-/-) mice. IHC on coronal sections of a mandibular incisor using indicated antibodies in wild-type (WT) (A, C, E, G, I, K, M, O, Q, U, W, Y) and mutant (B, D, F, H, J, L, N, P, R, V, X, Z) mice at E16.5 (U-Z) and E18.5 (A-R). All sections were counterstained with DAPI. Amelogenin- (A-F), Ameloblastin- (G-L), and Enamelin- (M-R) expressing ameloblasts were severely reduced in mutant mice. A secondary ameloblast-like layer was present on the lingual side of the mutant mice (white arrows in D, J, and P). Ameloblasts were reduced in size (white arrows in B, H, and N) with deformed or absent adhesion points on the labial side of incisors in the mutant mice (white stars in F, H, L, N, and R). (S) RT-quantitative PCR (RT-qPCR) analyses comparing levels of Amelogenin, Ameloblastin, Enamelin, and Laminin 5-3a expression in mandibular tissue of WT and mutant mice at E18.5. Expression levels of all 4 genes were significantly decreased in the mutants (P < 0.05 in all cases). (T) ChIP assays on proximal and distal promoter regions of the indicated genes conducted using qPCR. The ratio of amplification products present in immunoprecipitates from WT and mutant mice was determined to indicate the specificity of the ChIP signal in WT mice. Data shown in A-R and U-Z are representative of 3 and 5 similar experiments, respectively. RT-qPCR and ChIP data presented in (S) and (T) represent averages from studies using tissue from 3 (ChIP) to 6 (RT-qPCR) independent mice of each genotype. (U and V) Decreased expression levels of amelogenin in mutant ameloblasts at E16.5. The white dotted line represents the boundary between ameloblasts and odontoblasts in (V). (W and X) Immunostaining with an anti--tubulin antibody and DAPI. Y and Z indicate the DAPI stained nuclei of the ameloblats indicated in the versions of (W) and (X), respectively. White dots in (W-Z) denote the ameloblast apical boundary. Nuclei of WT ameloblasts were predominantly found on the basal surface (position labeled 1 in Y) or in the middle of the cell (position 2), but rarely on the apical aspect (position 3). In contrast, nuclei were randomly distributed throughout the mutant ameloblasts (positions 1-3 in Z). [Scale bars: (A-R) 200 um; (U-X) 100 um; (Y and Z) 50 um.]

Copyright

This image is from Golonzhka O, Proc Natl Acad Sci U S A 2009 Mar 17;106(11):4278-83. Copyright 2009 National Academy of Sciences, U.S.A. J:146765

Associated Alleles

Symbol	Name
Bcl11b^tm1.2Leid	B cell leukemia/lymphoma 11B; targeted mutation 1.2, Mark Leid

Associated Genotypes

Allelic Composition	Genetic Background
Bcl11b^tm1.2Leid/Bcl11b^tm1.2Leid	involves: ICR