Phenotype Image Detail

Image

Caption

In situ hybridization analysis show altered expression of left determinant genes in Megf8^hlb611/Megf8^hlb611 embryos. (A and B) Nodal is expressed at the node (arrowhead) and in the left LPM (arrow) in 4 somite stage wild-type (WT) embryo (A). In mutant embryos, Nodal expression was preserved at the node (arrowhead), but absent in the LPM. (C and D) In WT embryos, Lefty1 and 2 are normally expressed in the floor plate (arrowhead) and left LPM (arrow), respectively (C), but, in the mutants, expression of both Lefty 1 and 2 are lost (D). (E and F) Cryptic expression in the node and LPM show no change in the mutants (F) vs. wild-type (E) embryos. (G-I). WT embryos show Pitx2 expression in the left LPM (arrow in G), but this is either lost (H) or bilateral (arrowheads in I) in mutant embryos. Pitx2 expression in the head mesenchyme is unchanged. (J and K). Gdf1 expression in the node and LPM is not affected in mutant (K) vs. WT (J) embryos. (A, B, E, and F) Dorsal view. (C, D, and G-J) Ventral view. WT, wild-type; left (L)-right (R) orientation indicated by double arrowheads. (Scale bar: 100 um.)

Copyright

This image is from Zhang Z, Proc Natl Acad Sci U S A 2009 Mar 3;106(9):3219-24. Copyright 2009 National Academy of Sciences, U.S.A. J:146805

Associated Alleles

Symbol	Name
Megf8^hlb611	multiple EGF-like-domains 8; heart, lung and blood 611

Associated Genotypes

Allelic Composition	Genetic Background
Megf8^hlb611/Megf8^hlb611	involves: C57BL/6J