Phenotype Image Detail

Image

Caption

Podocyte foot process effacement in Nphs2^tm2.1Antc/Nphs2^tm3.1Antc Tg(CAG-cre/Esr1*)86Lbgn/0 mice. (A) Ultrastructural studies showed regular foot processes (FP) in healthy control animals on the opposite side of endothelial cells (En) lining the capillary lumen (L). (B) Irregularly shaped or fused FPs in mutant mice one week after induction (arrow). (C and D) Progression of focal changes to global fusion of FPs in mutant animals over time (Magnification, X10000). 3D modelling of glomerular structure showed no glomerular basement membrane (GBM) denudation in mutant animal (F) compared to controls (E). Blue colour represents GBM, pink and green represent FPs of adjacent podocytes. Severely affected FP number and organization in mutant animals (G). GBM thickening in mutant animals (H) (G and H: analysis is based on 3 animals per group). **p<0.01, ****p<0.0001.

Copyright

This image is from Tabatabaeifar M, PLoS One 2017;12(10):e0186574, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:245693

Associated Alleles

Symbol	Name
Nphs2^tm2.1Antc	nephrosis 2, podocin; targeted mutation 2.1, Corinne Antignac
Nphs2^tm3.1Antc	nephrosis 2, podocin; targeted mutation 3.1, Corinne Antignac
Tg(CAG-cre/Esr1*)86Lbgn	transgene insertion 86, Laurence Bugeon

Associated Genotypes

Allelic Composition	Genetic Background
Nphs2^tm2.1Antc/Nphs2^tm3.1Antc Tg(CAG-cre/Esr1*)86Lbgn/0	involves: 129S2/SvPas * C57BL/6 * DBA