Phenotype Image Detail

Image

Caption

TRPC3 is a critical regulator of dendritic growth and differentiation in cerebellar Purkinje cells. (A-D) In situ hybridization images for Trpc3 in wild-type (WT) mouse cerebellum at 8 days (A), 13 days (B), 18 days (C), and 56 days (D) of age. (E and F) Cerebellar sections of 3-week-old WT (E) and Trpc3^Mwk/Trpc3⁺ (F) mice subjected to immunohistochemistry using an anti-calbindin antibody. (G-J) Organotypic slice cultures (P8 + 12) prepared from WT mice (G) and mutant (H) littermates were stained with an anti-calbindin antibody. Arrowheads indicate dendritic trees. Mutant Purkinje cells had a significantly shorter longest dendrite (I) (44.8 +/- 2.5 um vs. 74.18 +/- 3.6 um) and significantly reduced areas of the dendritic arborization (J) (1358.7 +/- 119.9 um² vs. 3487.9 +/- 225.14 um²). Results shown are representative of 3 independent experiments (mean +/- SEM, n = 59, P < 0.0001, ANOVA followed by Fisher's PLSD post hoc test).

Copyright

This image is from Becker EB, Proc Natl Acad Sci U S A 2009 Apr 21;106(16):6706-11. Copyright 2009 National Academy of Sciences, U.S.A. J:148340

Associated Alleles

Symbol	Name
Trpc3^Mwk	transient receptor potential cation channel, subfamily C, member 3; moonwalker

Associated Genotypes

Allelic Composition	Genetic Background
Trpc3^Mwk/Trpc3^+	involves: BALB/cAnN * C3H/HeH