Phenotype Image Detail

Image

Caption

Hippocampal disorganization, increased c-fos, and loss of SOM+ interneurons in Dcx^tm1Caw/Y Dclk2^tm1.2Jgg/Dclk2^tm1.2Jgg (Dcx -/y ; Dclk2 -/-) mice. (A and B) DAPI-stained P18 coronal hippocampus in control delineate densely packed GCL (g.c.l.) in the dentate gyrus (D.G.) and pyramidal layer (p.l.) in the CA field. In the mutants the p.l. is delaminated in CA3 (arrow) and CA1 (arrowheads). The g.c.l. is also less packed and consequently thicker (Insets, compare brackets); n = 4. (C-F) Mutant pyramidal neurons show higher levels and more c-fos-positive cells than controls in both CA2/3 (arrows in C and E) and CA1 (arrowheads in D and F) fields at P18; n = 2. (G-J) SOM+ interneurons in both the D.G. hilus and the strata oriens (s.o.) of the CA region. Mutants show reduced number of SOM+ interneurons (30.44 +/- 7.48 vs. 64.56 +/- 6.74; P = 0.0276; n = 3 mice each), both in the D.G. (5.00 +/- 0.51 vs. 16.22 +/- 2.47; P = 0.0113), and in s.o. (15.89 +/- 5.73 vs. 40.78 +/- 4.64; P = 0.0279) (H and I). Remaining SOM+ interneurons express the same level of SOM (Inset in G and H). No significant difference in the total number of Parv+ (30.67 +/- 1.67 in mutants vs. 36.33 +/- 6.33 in controls; n = 2 mice of each genotype) or CaBP+ (63.15 +/- 14.58 in mutants vs. 59.44 +/- 7.99 in controls; n = 3 mice each); s.r., strata radiata; l.m., lacunosum moleculare. Dashed line delineates the hippocampal fissure. [Scale bars, 400 um (A, B, G, and H); 110 um (C-F).]

Copyright

This image is from Kerjan G, Proc Natl Acad Sci U S A 2009 Apr 21;106(16):6766-71. Copyright 2009 National Academy of Sciences, U.S.A. J:148345

Associated Alleles

Symbol	Name
Dclk2^tm1.2Jgg	doublecortin-like kinase 2; targeted mutation 1.2, Joseph G Gleeson
Dcx^tm1Caw	doublecortin; targeted mutation 1, Christopher A Walsh

Associated Genotypes

Allelic Composition	Genetic Background
Dclk2^tm1.2Jgg/Dclk2^tm1.2Jgg Dcx^tm1Caw/Y	involves: 129/Sv * C57BL/6