Phenotype Image Detail

Image

Caption

Morphological defect of Dcx^tm1Caw/Y Dclk2^tm1.2Jgg/Dclk2^tm1.2Jgg (Dcx -/y ; Dclk2 -/-) hippocampal pyramidal cells in vivo and in vitro. (A-F) Golgi-Cox-stained hippocampus at P11 showing mutants with less secondary and tertiary apical pyramidal dendrites (D-F) (6.06 +/- 0.48; n = 32 neurons, n = 2 mice) than controls (A and F) (10.09 +/- 0.39; n = 53 neurons in n = 3 mice; P < 0.001). Dendrite number in Dcx (B and F) (9.46+/- 0.48 n = 40 neurons in n = 2 mice) or Dclk2 (9.06 +/- 0.57; n = 35 neurons in n = 2 mice) single knockouts is normal. Total number of basolateral dendrites is normal in all (F) (10.33 +/- 0.69 in Dcx; Dclk2 nulls, 11.53 +/- 0.73 in Dcx nulls, 11.88 +/- 1.12 in Dclk2 nulls vs. 9.71 +/- 0.48 in controls; n = 32-53 neurons, n = 3 control mice and n = 2 mice each). (G-J) Neurite outgrowth of Dcx; Dclk2 null pyramidal cells is greatly reduced compared with controls. Both the total number of neurites (I) (13.95 +/- 1.08 in Dcx; Dclk2 nulls vs. 23.67 +/- 2.92 in control; P = 0.0006; n = 21 and 9 neurons) and total length of neurites (J) (967.56 +/- 119.12 in Dcx; Dclk2 nulls vs. 1809.44 +/- 179.02 in control; P = 0.0006; n = 21 and 9 neurons; n = 2 mice each) are significantly reduced. [Scale bars, 40 um (A-E).]

Copyright

This image is from Kerjan G, Proc Natl Acad Sci U S A 2009 Apr 21;106(16):6766-71. Copyright 2009 National Academy of Sciences, U.S.A. J:148345

Associated Alleles

Symbol	Name
Dclk2^tm1.2Jgg	doublecortin-like kinase 2; targeted mutation 1.2, Joseph G Gleeson
Dcx^tm1Caw	doublecortin; targeted mutation 1, Christopher A Walsh

Associated Genotypes

Allelic Composition	Genetic Background
Dclk2^tm1.2Jgg/Dclk2^tm1.2Jgg Dcx^tm1Caw/Y	involves: 129/Sv * C57BL/6