Phenotype Image Detail

Image

Caption

Dyslaminated interneuron populations in Dcx^tm1Caw/Y Dclk2^tm1.2Jgg/Dclk2^tm1.2Jgg (Dcx -/y ; Dclk2 -/-) and Dcx^tm1Caw/Y (Dcx -/y) mice. (A-C) Total number of SOM+ interneurons is similar in control (A and C) (72.67 +/- 8.50; n = 3 mice) and Dcx; Dclk2 nulls (B and C) (61.71 +/- 14.96; n = 2 mice). Level of SOM+ innervation by the O-LM cells to the l.m. is similar in the control and Dcx; Dclk2 null hippocampus (asterisks in A and B). (D-I) Total number of CaBP+ interneurons is normal at this stage (D) (50.54 +/- 6.97 in Dcx; Dclk2 nulls vs. 60.10 +/- 7.75 in controls; n = 5 controls, 3 nulls). However, the location of the CaBP+ interneurons within the CA field is abnormal in Dcx -/y and Dcx; Dclk2 nulls. In control (E and F) and Dclk2 -/- (E and H), the number of CaBP+ interneurons located at the limit between the s.r. and l.m. (limit, white bracket) is greater than the number of interneurons located within the rest of the s.r. (the ratio limit/s.r. are 1.73 +/- 0.14 and 1.79 +/- 0.25, respectively). This ratio is significantly lower in Dcx -/y (E and G) (0.86 +/- 0.10; P = 0.0152 to control) or Dcx; Dclk2 nulls (E and I) (0.74 +/- 0.15; P = 0.0103 to control) hippocampus (n = 5 control, 2 of each single null and 3 double nulls). [Scale bars, 400 um (A and B); 50 um (F-I).]

Copyright

This image is from Kerjan G, Proc Natl Acad Sci U S A 2009 Apr 21;106(16):6766-71. Copyright 2009 National Academy of Sciences, U.S.A. J:148345

Associated Alleles

Symbol	Name
Dclk2^tm1.2Jgg	doublecortin-like kinase 2; targeted mutation 1.2, Joseph G Gleeson
Dcx^tm1Caw	doublecortin; targeted mutation 1, Christopher A Walsh

Associated Genotypes

Allelic Composition	Genetic Background
Dclk2^tm1.2Jgg/Dclk2^tm1.2Jgg Dcx^tm1Caw/Y	involves: 129/Sv * C57BL/6
Dcx^tm1Caw/Y	involves: 129/Sv * C57BL/6