Phenotype Image Detail

Image

Caption

Erythropoietic defects in fetal liver of Senp1^tm1.1Wami/Senp1^tm1.1Wami (KO) mice. (A) Appearance of wild-type (WT) and KO embryos at E16.5. Freshly dissected embryos without staining were photographed. Embryo with yolk sac (top) and without yolk sac (bottom) are shown. Yolk sac, placenta, and fetal liver are indicated. Bars, 200 uM. (B) Wet weight of fetal livers in WT and KO embryos (n = 6 each group). Data are mean SEM from five fetal livers per group. *, P < 0.01. (C) Histology examination of WT and KO E13.5 fetal livers by hematoxylin/eosin staining. Bars, 100 uM. (D) Number of erythropoietic cells per high-power field (40X) was quantified from five sections. *, P < 0.01. (E) Freshly isolated E13.5 WT or KO fetal liver cells were incubated in PharmLyse to remove mature red blood cells, and the remaining erythroid progenitor cells were doubly stained with anti-Ter119 followed by flow cytometry (FACS) analysis. The percentage of Ter119+ cells is shown. Data are mean SEM from five fetal livers per group. *, P < 0.01. (F) E13.5 fetal liver touch preparations and May-Giemsa staining. Arrows indicate proerythroblasts. Bars, 20 uM.

Copyright

This image is from Yu L, J Exp Med 2010 Jun 7;207(6):1183-95, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:163428

Associated Alleles

Symbol	Name
Senp1^tm1.1Wami	SUMO1/sentrin specific peptidase 1; targeted mutation 1.1, Wang Min

Associated Genotypes

Allelic Composition	Genetic Background
Senp1^tm1.1Wami/Senp1^tm1.1Wami	involves: 129 * C57BL/6