Phenotype Image Detail

Image

Caption

Assessment of atherosclerosis initiation and progression in atheroprone male and female mice with or without endothelial-specific deletion of NCK2. APOE-deficient (Apoe^tm1Unc/Apoe^tm1Unc) mice were crossed with mice homozygous for both an Nck1-null allele and a floxed Nck2 allele and that carried inducible cre recombinase to selectively inactivate Nck2 in the endothelium (Tg(Cdh5-cre/ERT2)1Rha/0 Nck1^tm1Paw/Nck1^tm1Paw Nck2^tm3Paw/Nck2^tm3Paw). Tamoxifen-induced cre recombination mediates excision of the loxP site-flanked exon 1 of Nck2. Experimental groups consisted of male and female mice with (eNck2+) and without (eNck2-) NCK2 expression in the endothelium. (E) Representative images of en face preparations of mouse thoracic aortas stained with Oil Red O. Aortas were obtained from male (left) and female (right) mice fed a high-fat diet (HFD) for 8 or 16 weeks to assess atherosclerosis initiation and progression, respectively. The scale bar represents 2 mm. (F) Box plots showing quantification of plaque burden as percentage of exposed intimal area occupied by lesions in male (left) and female (right) mice. Statistical significance was determined by two-way ANOVA followed by Tukey's multiple means comparison test. *p<0.05. Numbers of animals/group (n) are indicated in parentheses.

Copyright

This image is from the Laboratory of Dr. Gonzalo M Rivera at Texas A&M University and is displayed with the permission of the author. J:327349

Associated Alleles

Symbol	Name
Apoe^tm1Unc	apolipoprotein E; targeted mutation 1, University of North Carolina
Nck1^tm1Paw	non-catalytic region of tyrosine kinase adaptor protein 1; targeted mutation 1, Tony Pawson
Nck2^tm3Paw	non-catalytic region of tyrosine kinase adaptor protein 2; targeted mutation 3, Tony Pawson
Tg(Cdh5-cre/ERT2)1Rha	transgene insertion 1, Ralf H Adams

Associated Genotypes

Allelic Composition	Genetic Background
Apoe^tm1Unc/Apoe^tm1Unc Nck1^tm1Paw/Nck1^tm1Paw Nck2^tm3Paw/Nck2^tm3Paw Tg(Cdh5-cre/ERT2)1Rha/0	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6