Phenotype Image Detail

Image

Caption

Determination of ICAM-1 in aortic roots of atheroprone mice with (Apoe^tm1Unc/Apoe^tm1Unc Tg(Cdh5-cre/ERT2)1Rha/0 Nck1^tm1Paw/Nck1^tm1Paw Nck2^tm3Paw/Nck2^tm3Paw) and without (Apoe^tm1Unc/Apoe^tm1Unc Tg(Cdh5-cre/ERT2)1Rha/0 Nck1^tm1Paw/Nck1^tm1Paw) endothelial-specific deletion of NCK2. Animals were fed a HFD for 8 or 16 weeks to assess atherosclerotic lesion initiation and progression, respectively. (A/C) Representative confocal images of aortic roots showing immunofluorescence staining of endothelial (CD31) and cell adhesion (ICAM-1) markers in males (A) and females (C). The dotted yellow, white, and green lines contour the aortic root section, intimal, and neointima layers, respectively. The scale bars represent 50 um unless otherwise indicated. (B/D) Box plots showing quantification of total (left panels), intimal (center panels), and neointimal (right panels) ICAM-1 in males (B) and females (D). Total ICAM-1was calculated as ICAM-1 area in entire root/root area. Intimal ICAM-1 was calculated as the area of ICAM-1/area of CD31 in signal in a 10 um-thick periluminal layer (intima). Neointimal ICAM-1 was calculated as area of ICAM-1/neointimal area. Dots represent individual animals and the number of animals per group is indicated in parentheses. Statistical significance was determined by Wilcoxon Rank Sum Test with Bonferroni correction. *p < 0.05.

Copyright

This image is from the Laboratory of Dr. Gonzalo M Rivera at Texas A&M University and is displayed with the permission of the author. J:327349

Associated Alleles

Symbol	Name
Apoe^tm1Unc	apolipoprotein E; targeted mutation 1, University of North Carolina
Nck1^tm1Paw	non-catalytic region of tyrosine kinase adaptor protein 1; targeted mutation 1, Tony Pawson
Nck2^tm3Paw	non-catalytic region of tyrosine kinase adaptor protein 2; targeted mutation 3, Tony Pawson
Tg(Cdh5-cre/ERT2)1Rha	transgene insertion 1, Ralf H Adams

Associated Genotypes

Allelic Composition	Genetic Background
Apoe^tm1Unc/Apoe^tm1Unc Nck1^tm1Paw/Nck1^tm1Paw Nck2^tm3Paw/Nck2^tm3Paw Tg(Cdh5-cre/ERT2)1Rha/0	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6