Phenotype Image Detail

Image

Caption

Polr1a^{tm1b(EUCOMM)Hmgu}/Polr1a^{tm1b(EUCOMM)Hmgu} embryos cannot be recovered at E7.5 (0/29), nor are there excess resorption sites. Homozygous mutants are recovered at E3.5 and appear as dying 2-cell stage embryos, suggesting zygotic genome activation failure. No further development occurs, with mutants never reaching a morphological blastocyst stage. RT-PCR indicates robust expression. Knock-in LacZ also shows robust expression in gastrulation stages. Additional information at websites.umass.edu/jmager/Polr1a

Copyright

This image was contributed directly to MGI. Questions regarding this image or these data should be directed to Dr. Jesse Mager at jmager@umass.edu J:279207

Associated Alleles

Symbol	Name
Polr1a^tm1b(EUCOMM)Hmgu	polymerase (RNA) I polypeptide A; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH

Associated Genotypes

Allelic Composition	Genetic Background
Polr1a^tm1b(EUCOMM)Hmgu/Polr1a^tm1b(EUCOMM)Hmgu	C57BL/6N-Polr1a^tm1b(EUCOMM)Hmgu/Bay