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| Caption | (d) Diagram illustrating the gene targeting for the EUCOMM pre-conditional Smoc1 knockout mouse, Smoc1tm1a(EUCOMM)Wtsi/Smoc1tm1a(EUCOMM)Wtsi, at the genomic level (top) with the relative positions of RT-PCR primers indicated. Targeting cassette (bottom) is specific for exon 4 of Smoc1. Positions of genotyping PCR primers are indicated (GT-F & -R2, blue, are endogenous-gene specific, GT-R1 is cassette-specific). Note that the targeting cassette has the beta-galactosidase ORF inserted downstream of an EN2 splice acceptor site (EN2 SA) and internal ribosomal entry site (IRES), allowing for reporter expression in successfully Smoc1-targeted animals (pA is Poly(A) tail). The cassette also features FRT and Cre-recombinase (loxP) sequences for future gene manipulation. Ensembl exon identifiers are given. | ||||||
| Copyright | This image is from Rainger J, PLoS Genet 2011 Jul;7(7):e1002114, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:174198 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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