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| Caption | (A) Schematic representation of the TRPM2 genomic targeting strategy using CRE-lox technology. Wild-type (WT) TRPM2 genomic locus displaying exons 16-22 and restriction sites (A, Apal; B, Bgl II; E, EcoRV; N, Ncol; and X, Xbal) are shown. Targeting vector containing 5' homology arm (~3.8 kb), neomycin resistance and thymidine kinase selection (neo'-TK) flanked by LoxP sites, the third LoxP within intron 20 and the 3' arm of homology (2.8 kb) flanked by diphtheria-toxin A (DTA) negative selection marker are represented. The resultant TRPM2 primary targeted locus following homologous recombination of the targeting construct demonstrates integration of the neo'-TK and LoxP sites. In vitro Cre-mediated recombination results in deletion of the neo'-TK cassette and exons 19 and 20 as depicted in the representation of the Trpm2tm1.1Alp (TRPM2delta19/20) locus. | ||||
| Copyright | This image is from Knowles H, Proc Natl Acad Sci U S A 2011 Jul 12;108(28):11578-83. Copyright 2011 National Academy of Sciences, U.S.A. J:174397 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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