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| Caption | Targeting vector to generate NFAT5 null mutant mice. (A) The genomic structure of mouse NFAT5 gene. Exons are represented in black boxes and numbered with 1-16. Introns are represented by lines. Targeting construct for Nfat5tm1Skc knockout allele which contains the thymidine kinase gene (tk) and the neomycin resistance gene (Neo). The targeting vector was linearized with Not1 restriction endonuclease. Exon 5 and 6 are shown in boxes and the restriction map of the endogenous NFAT5 and the mutant Nfat5tm1Skc allele after homologous recombination are shown. The primers (ptkR, pKB2.5T7R, p3.pA and p3RA) used for PCR screening are shown as arrows. For Southern hybridization screening, 600-bp Spe1-BamH1 fragment was used as external probe, the expected sizes of EcoRV fragments from wild type allele and homologous recombinant were 7.5 kb and 4.5 kb respectively. E: EcoRV, B: BamH1, K: Kpn1, N: Not1, S: Spe1. | ||||
| Copyright | This image is from Mak MC, PLoS One 2011;6(7):e19186, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:175527 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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