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| Caption | Generation of the Tcrbtm8.1Slek (TcrbF) allele. Shown is a schematic of the Cb2 region of the Tcrbtm8.1Slek allele (top) and the pLNTK-Cb2F targeting vector used to generate the Tcrb allele with the Neo cassette (TcrbF+Neo), which has a targeted replacement of the four Cb2 exons with a DNA fragment containing a fusion of these exons and the loxP-flanked neomycin resistance gene. Also shown is the Tcrbtm9.1Slek allele generated after Cre-mediated deletion of the neomycin resistance gene, leaving a single loxP site. The relative positions of the different restriction sites are shown, as are probes A and B, which were used for Southern blot analyses. Southern blots of genomic DNA from targeted ES cell lines that were digested with PstI and hybridized to probe B, or digested with SacI and hybridized to probe A are shown. The molecular weight markers and relative position of the bands generated by the different Tcrb alleles are indicated. | ||||
| Copyright | This image is from Mahowald GK, PLoS One 2011;6(7):e21627, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:176070 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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