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| Caption | Generation of the Tcrbtm8.1Slek (TcrbA) allele. Shown is a schematic of part of the Tcrb allele in which the Db1 gene segment has been deleted (top, Jb1M3 in ref. [28]). The Db and Jb gene segments are shown as open rectangles (except for Jb2.3, shown as a shaded rectangle) and the RSs as open triangles. The Cb1 and Cb2 exons (blue rectangles) are also shown, as is the pLNTK-DJ targeting vector used to generate the Tcrb allele with the Neo cassette (TcrbA+Neo), which has a targeted replacement of the Db2, Jb2.1, Jb2.2 and Jb2.3 gene segments with a Db2Jb2.3 rearrangement and the loxP-flanked neomycin resistance gene (NeoR). The Tcrbtm8.1Slek (TcrbA) allele generated after Cre-mediated deletion of the neomycin resistance gene, leaving a single loxP site (filled oval), is shown. The relative positions of the different restriction sites are shown, as is probe A, which was used for Southern blot analysis of the different targeted alleles. Also shown is a Southern blot of genomic DNA from targeted ES cell lines digested with SacI and hybridized to probe A. The molecular weight markers and relative positions of the bands generated by the different Tcrb alleles are indicated. | ||||
| Copyright | This image is from Mahowald GK, PLoS One 2011;6(7):e21627, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:176070 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/17/2024 MGI 6.24 |
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