Molecular Image Detail

Image

Caption

Emu deletion and VH gene insertion in an Igh^a locus to generate the Igh^tm1Leck allele. (A) Targeting strategy. WT^b, WT Igh^b locus. Thick vertical bars show exons for DQ52, JH1-4, and the first two exons of Cmu. Smu, mu switch region; shaded oval, Emu; B, BamHI; H, HindIII; WT^a, unmodified Igh^a locus. Shaded boxes (5'H and 3'H) show regions of homology with targeting vector. Targeting vector: LoxPNeoR, loxP-flanked neomycin resistance gene (white arrowheads indicate loxP sites), B1-8VH promoter region (shaded), and coding sequences (thick vertical lines). neoRVHdelta^a:Igh^a allele after homologous recombination. Igh^tm1Leck (VHdealta^a), modified Igh^a allele after neoR gene deletion.

This image is from Li F, J Exp Med 2009 Jan 16;206(1):153-67, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:144033

Associated
Alleles

Symbol	Name
Igh^tm1Leck	immunoglobulin heavy chain complex; targeted mutation 1, Laurel A Eckhardt

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