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| Caption | (A) Schematic representation of Mir146 knock-out (KO) targeting strategy to generate the Mir146tm1.1Bal and Mir146tm2.1Bal alleles. To ablate Mir146 expression, we replaced a 295-bp fragment, containing all of the Mir146 mouse precursor sequence, with a Neomycin resistance (Neo R) cassette flanked by loxP sites by homologous recombination in embryonic stem cells. The Neo R cassette was then floxed out by mating F1 generation heterozygous mice with germline-expressing Cre recombinase transgenic animals, Tg(EIIa-cre)C5379Lmgd/0, resulting in a swap of mutant precursor sequence for a single loxP site. The locations of the 3' and 5' end Southern probes and PCR primers (P1 and P2) are depicted in the graph. B, BamHI; DTA, diphtheria toxin; K, KpnI. | ||||||
| Copyright | This image is from Boldin MP, J Exp Med 2011 Jun 6;208(6):1189-201, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:173671 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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