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| Caption | (A) Generation of the Gt(ROSA)26Sortm3(CAG-Il17a)Awai and Gt(ROSA)26Sortm3.1(CAG-Il17a)Awai alleles was carried out using homologous recombination in embryonic stem cells. Our conditional 'knock-in' approach introduced the targeting construct into the endogenous Gt(ROSA)26Sor locus to generate the Gt(ROSA)26Sortm3(CAG-Il17a)Awai allele. Upon Cre-mediated recombination, a lox-P-flanked transcriptional STOP cassette is excised 5' of an Il17a cDNA insert and an IRES-EGFP element, allowing a dual expression of Il17a and EGFP under the control of the chicken Beta-actin (CAG) promoter. Mating of mice expressing the Gt(ROSA)26Sortm3(CAG-Il17a)Awai allele with germ-line Cre-expressing mice results in the Gt(ROSA)26Sortm3.1(CAG-Il17a)Awai allele. | ||||||
| Copyright | This image is from Haak S, J Clin Invest 2009 Jan;119(1):61-9 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:144710 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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