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Caption | A, the targeting construct used to disrupt the endogenous Lzts2 gene in ES cells is shown in the figure. Briefly, a PGK neomycin cassette flanked by loxP and FRT sites was inserted upstream of exon 2 containing the translation start codon. A third loxP site was inserted downstream of exon 3 using Red/ET recombineering. The hypothetical crossovers between the endogenous Lzts2 allele and the targeting construct are indicated by the dashed lines. Correctly recombined ES clones were identified by PCR using primer sets for the Neo cassette (Neo1), flanking sequences (P1), and the downstream loxP site (P3/P4). The desired disruption of Lzts2 to generate the Lzts2tm1.1Zsu allele can be induced by the Cre/loxP system. | ||||
Copyright | This image is from Peng Y, J Biol Chem 2011 Nov 18;286(46):40331-42 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:178154 | ||||
Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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