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| Caption | Generation of the Cdh5tm4(Cdh5/Fkbp,Ptprb/Mtor*)Dvst allele. (A) Schematic illustration of the recombination-mediated cassette exchange (RMCE) approach. The gene replacement cassette contains cDNAs for Cdh5 fused with Fkbp (VE-cadherin-FKBP) and full-length Ptprb fused to a mutated form of the rapamycin binding domain of mammalian target of rapamycin (VE-PTP-FRB*) separated by an internal ribosome entry site (IRES), as well as a Hygromycin cassette, flanked by two FRT sites. The cassette is flanked by two incompatible loxP sites. Using RMCE, the ATG-containing exon 2 of Cdh5 was replaced by the cDNA insertion cassette. Positions of the primers for PCR-genotyping are indicated. | ||||
| Copyright | This image is from Broermann A, J Exp Med 2011 Nov 21;208(12):2393-401, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:178759 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/10/2024 MGI 6.24 |
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