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| Caption | (A) To generate a loxP-tagged Npy1rtm1.1Ceva (Npy1rloxP) allele, we inserted loxP sites around exons 2-3, which cover the entire Npy1r coding region, using standard gene targeting techniques in embryonic stem (ES) cells. The neo cassette was removed in vivo by crossing the Npy1rloxPneo mice to flp transgenic mice carrying the flippase (flp) recombinase, which recognizes and cuts at the frt sites and generates the Npy1rtm1.1Ceva allele. Mice withe the Npy1rtm1.1Ceva allele were crossed with cre-deleter transgenic mice, removing exon 2 and 3 and generating the Npy1rtm1.2Ceva (Npy1r-) allele. Light gray boxes represent exons. Dark gray boxes are the coding regions with, in black, the transmembrane domains. B, BglII; E, EcoRV; K, KpnI; M, MscI; P, PstI; S, SphI. Frt and loxP sites are in blue and red triangles, respectively. Circles indicate the B and K restriction sites, which are borders of the targeting vector. Arrows indicate the size of genomic fragments generated on Southern blotting by cleavage with MscI. | ||||||
| Copyright | This image is from Bertocchi I, Proc Natl Acad Sci U S A 2011 Nov 29;108(48):19395-400. Copyright 2011 National Academy of Sciences, U.S.A. J:180396 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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