Molecular Image Detail

Image

Caption

(A) Schematic of the Pold1 domain structure and locations of the modifications with a Pold1 gene targeting method. (B) Structure of the targeting vector and partial restriction map of the murine Pold1 locus before and after homologous recombination (Pold1^tm1Uchi; Pold1^-) and Cre mediated recombination (Pold1^tm1.1Uchi; Pold1^exo). Exons are represented by vertical black boxes and introns by intervening horizontal lines. *indicates D400A substitution. The genomic fragment used as a probe for Southern blotting is indicated by the horizontal blue bar. Restriction enzymes: D = DraI; N = NehI. PCR primers P0 and P2 were used to screen for homologous recombinant ES cells. PCR primers P1, P2 and P3 were used for genotyping.

This image is from Uchimura A, PLoS ONE 2009;4(1):e4184, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:144992

Associated
Alleles

Symbol	Name
Pold1^tm1.1Uchi	polymerase (DNA directed), delta 1, catalytic subunit; targeted mutation 1.1, Arikuni Uchimura
Pold1^tm1Uchi	polymerase (DNA directed), delta 1, catalytic subunit; targeted mutation 1, Arikuni Uchimura

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