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| Caption | The 5' and 3' arms of the alphaA-crystallin gene (Cryaa) were cloned into a vector containing the floxed neomycin (neo) cDNA. Mutagenesis was performed to mutate amino acid arginine 49 to cysteine (R49C). The asterisk above exon 1 indicates the mutation. The numbered blue rectangles indicate exons. The filled triangles are loxP sites and X denotes the XhoI site. Mouse embryonic stem (ES) cells SCC-10 were electroporated with the mutant plasmid, and clones testing positive for neo were identified and used to generate heterozygous Cryaatm1Ady mice. Cre-mediated recombination in the germline removes the neo cassette (mutated allele without neor) and generates the Cryaatm1.1Ady allele. | ||||||
| Copyright | This image is from Andley UP, BMC Ophthalmol 2009;9():4, an open-access article, licensee BioMed Central Ltd. J:157274 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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