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| Caption | (A,B) A Cre-ERT2-IRES-EYFP-Neo construct was introduced in frame with ATG of exon 1 by homologous recombination. Genes coding for Neomycin resistance (Neo) and Diphtheria Toxin A were used for positive and negative selection respectively. (C) Chimeras carrying the Neo cassette were crossed with C57BL/6J mice ubiquitously expressing Flp recombinase to generate heterozygous knock-in mice lacking the Neo cassette (D). Primers used for genotyping are shown as black arrows. Note that primers P1 and P2 detect the wild-type locus (297 bp band) whereas primers P3 and P4 detect the recombined locus (271 bp band). C: control; DTA: Diphtheria Toxin A; pGK: Phosphoglycerate kinase promoter. | ||||
| Copyright | This image is from El Agha E, PLoS One 2012;7(6):e38452, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:187831 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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