Molecular Image Detail

Image

Caption

Generation of the floxed, Huwe1^tm1.1Mak, allele (floxed Mule) and knock-out Huwe1^tm1.2Mak allele. (A) Targeting scheme. (1) Restriction map of the wild-type (WT) genomic mouse Huwe1 locus. Probes used to verify targeting events are indicated as a, b, and c. The expected sizes of the restriction fragments are shown. B, BamHI; S, StuI. (2) Targeting vector (TV) construction. The loxP-flanked neomycin resistance gene (neo) was used as a selection marker during ES cell culture. Exons 76 and 77, which encode the HECT domain essential for Huwe1's E3 ubiquitin ligase activity, were flanked by loxP sites (triangles). (3-5) The structure of the targeted Huwe1 locus (3), the targeted locus after removal of neo (Huwe1^tm1.1Mak) (4), and the locus after deletion of the floxed exons (Huwe1^tm1.2Mak)(5) are shown, including the sizes of diagnostic restriction fragments.

This image is from Hao Z, J Exp Med 2012 Jan 16;209(1):173-86, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:181705

Associated
Alleles

Symbol	Name
Huwe1^tm1.1Mak	HECT, UBA and WWE domain containing 1; targeted mutation 1.1, Tak Mak
Huwe1^tm1.2Mak	HECT, UBA and WWE domain containing 1; targeted mutation 1.2, Tak Mak

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 11/12/2024 MGI 6.24