About   Help   FAQ
Molecular Image Detail
Image
Caption A, strategy for disrupting the mouse Kcne3 gene. Top model, wild-type (WT) allele with exons depicted as boxes. Exon 4 contains the entire coding region. Lower models, targeting construct containing a neomycin cassette (neo) as positive and the diphtheria toxinA cassette (dta) and thymidine kinase (tk) as negative selection markers. The neomycin cassette was flanked by loxP sites (black triangle). A third loxP site was inserted after exon 4. In the recombined allele, the neomycin cassette introduced a new BglI restriction site, giving rise to 6- and 8-kb BglI restriction fragments. This results in the floxed Kcne3tm1Tjj allele. The Kcne3tm1.1Tjj allele (bottom) is obtained by Cre-recombinase mediated excision of exon 4. A 9.5-kb BglI restriction fragment (that is shorter than the 12-kb fragment of the WT allele) may be detected with 5' and 3' probes (bars at bottom) by Southern blotting.
Copyright This image is from Preston P, J Biol Chem 2010 Mar 5;285(10):7165-75 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:160721
Associated
Alleles
Symbol Name
Kcne3tm1.1Tjj potassium voltage-gated channel, Isk-related subfamily, gene 3; targeted mutation 1.1, Thomas J Jentsch
Kcne3tm1Tjj potassium voltage-gated channel, Isk-related subfamily, gene 3; targeted mutation 1, Thomas J Jentsch

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
10/29/2024
MGI 6.24
The Jackson Laboratory