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| Caption | A, an embryonic stem cell line containing a GeneTrap targeting vector in intron 1 of the Egln1 (Hif-p4h-2) gene, disrupting the endogenous transcription and leading to the production of truncated mRNA coding for beta-galactosidase, was used to generate a Egln1Gt(RRG405)Byg/Egln1Gt(RRG405)Byg (Hif-p4h-2gt) mouse line. The arrows represent the primers used in the genotyping by PCR and in RT-PCR. The probe used in Southern blotting is depicted as a black bar. E1-E5, exons 1-5; E, EcoRI; SA, splice acceptor sequence; beta-geo, a fusion gene formed from the beta-galactosidase and neomycin resistance genes; pA, polyadenylation signal. | ||||
| Copyright | This image is from Hyvarinen J, J Biol Chem 2010 Apr 30;285(18):13646-57 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:162597 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/17/2024 MGI 6.24 |
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