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Caption A, in the Fbn1 (GT8) targeting vector, a poly(A) tail, eGFP, and a neomycin selection cassette (PGK-Neo, flanked by FRT sites) were placed in the intron between exons 34 and 35. A polyglycine linker (GGGG) and exon 33 splice acceptor site were engineered 5' to the inverted coding region for eGFP. Cre-mediated recombination of special lox sites (lox66 and lox77) resulted in the inversion of eGFP behind exon 32, separated from exon 32 by the polyglycine linker. The neomycin selection cassette was removed after breeding to Flpe transgenic mice, generating the Fbn1tm1.2Lysa allele.
Copyright This image is from Charbonneau NL, J Biol Chem 2010 Aug 6;285(32):24943-55 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:165897
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Alleles
Symbol Name
Fbn1tm1.2Lysa fibrillin 1; targeted mutation 1.2, Lynn Y Sakai

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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory