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Caption (A) Targeting strategy. Top: wild-type (wt) allele containing the Airn promoter, below: targeting vector that deletes the TDRs (grey bar) lying in the 3' part of the CGI (green bar) with homology regions indicated. A floxed selection cassette (Pgk1 promoter driving a neomycin resistance gene (neo) stopped by a Pgk1 polyadenylation signal (pA); flanked on both sides by loxP sites) was inserted into the NheI site of Igl2r intron 3. Homologous recombination created the TDRdelta+cas allele. ES cells with a TDRdelata+cas allele were injected into blastocysts, mice carrying a targeted allele were identified and the selection cassette was removed by mating with a MORE-CRE expressing mouse to generate the Airntm2.1Dpb allele. Note the remaining loxP site in the mutant allele lies 1.3 kb upstream of the Airn promoter (black triangle).
Copyright This image is from Koerner MV, PLoS Genet 2012 Mar;8(3):e1002540, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:183397
Associated
Alleles
Symbol Name
Airntm2.1Dpb antisense Igf2r RNA; targeted mutation 2.1, Denise P Barlow

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last database update
11/19/2024
MGI 6.24
The Jackson Laboratory