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| Caption | Targeting strategy for generation of Gata4tm1Grg knock-in allele. (A) Single nucleotide change resulting in the glycine to serine mutation was introduced into the mouse Gata4 locus. Partial restriction map of the murine Gata4 wild-type allele (top), the Gata4 targeting vector (middle), and successfully targeted allele, Gata4tm1Grg,(bottom) are shown. Homologous recombination results in replacement of wild-type Gata4 with genomic DNA harboring a substitution of glycine to serine at position 295 into the mouse Gata4 locus, as well as the incorporation of neomycin cassette surrounded by loxP sites. Gata4 coding exons are shown as empty boxes, whereas the exon used as a probe used for Southern blot analysis is highlighted by a black bar. NZf, amino- terminal zinc finger (exon 2); CZf, carboxy- terminal zinc finger (exon 3); E4, exon 4; E5, exon 5; E6, exon 6; B, BglI; S, SacI; E, EcoRV; and N, NotI. | ||||
| Copyright | This image is from Misra C, PLoS Genet 2012 May;8(5):e1002690, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:185124 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 03/25/2025 MGI 6.24 |
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