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| Caption | Generation of Clcn5tm4.1Tjj (clcn5Y672E) knock-in allele. A, Targeting strategy. Top, the targeting construct containing 12.5 kb of mouse genomic Clcn5 sequence was modified by inserting the Y672E mutation into exon 11. For positive selection, a FRT flanked neomycin-resistance cassette was inserted into intron 11 and for negative selection, a diphtheria toxin A (dta) cassette was added at the 5' end. In addition, an Acc651 (A) site (with a loxP site) was inserted into intron 9 and an EcoRV site was added in the 3' UTR for screening purposes (A and B). In vivo Flp-mediated deletion of the neomycin resistance cassette resulted in the Clcn5tm4.1Tjj (clcn5Y672E) allele. | ||||
| Copyright | This image is from Rickheit G, J Biol Chem 2010 Jun 4;285(23):17595-603 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:164474 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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