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Caption (a) Schematic representation of the R26NZG targeting strategy. A reporter cassette composed of the CAG promoter, a floxed (orange triangles) PGKNEO-4pA cassette, an nlslacZ-3pA cassette flanked by FRT sites (purple double triangles), and EGFP-pA was knocked into the ROSA(26)Sor locus. 5' and 3' homology arms are shown as bold lines. B, BamHI; X, XbaI. (b) Schematic diagram of Cre- and FLP-dependent recombination and derivative reporter alleles. R26NZG (Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh) is a Cre-dependent nlslacZ reporter that can be converted to a Cre-dependent EGFP reporter, R26NG (Gt(ROSA)26Sortm1.2(CAG-EGFP)Glh), by FLP-dependent excision of the nlslacZ cassette. Germ line excision of the PGKNEO cassette by Cre converts R26NZG (Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh) into a FLP-dependent EGFP reporter, R26ZG (Gt(ROSA)26Sortm1.1(CAG-lacZ,-EGFP)Glh), which expresses nlslacZ in the absence of FLP. By the simultaneous use of appropriate Cre and FLP deleters, R26NZG also functions as a dual reporter that expresses nlslacZ after Cre-mediated recombination and EGFP only after both Cre- and FLP-mediated recombination. The transcriptionally active genes are colored blue (nlslacZ), green (EGFP), or red (PGKNEO) in (b).
Copyright This image is from Yamamoto M, Genesis 2009 Feb;47(2):107-14, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:147304
Associated
Alleles
Symbol Name
Gt(ROSA)26Sortm1.1(CAG-lacZ,-EGFP)Glh gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, David J Goldhamer
Gt(ROSA)26Sortm1.2(CAG-EGFP)Glh gene trap ROSA 26, Philippe Soriano; targeted mutation 1.2, David J Goldhamer
Gt(ROSA)26Sortm1.3(CAG-EGFP)Glh gene trap ROSA 26, Philippe Soriano; targeted mutation 1.3, David J Goldhamer
Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh gene trap ROSA 26, Philippe Soriano; targeted mutation 1, David J Goldhamer

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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory