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| Caption | Targeted gene replacement of adult mouse beta globin genes with a human gamma to beta0 globin switching cassette. A, schematic demonstration of gene replacement in the mouse beta globin locus (Hbb) tagged with human gamma beta globin and hprt genes with gamma beta0 globin and hyg genes. B, schematic demonstration of the wild-type mouse beta globin locus and gamma beta0 KI allele, Hbbtm1.1(HBG1,HBB*)Ryan, after marker gene removal with labeled restriction endonuclease sites that confirm correct homologous recombination. The 5' probe anneals to a 9.2-kb XbaI fragment from the wild-type allele and a 6.9-kb XbaI fragment from the human gamma beta0 globin KI allele. The 3'-probe derived from part of the betamin globin gene, anneals to a 14.8-kb EcoRI fragment from the betamin globin gene, a 7.3-kb fragment from the betamaj globin gene, and a 10.3-kb fragment from the gamma beta0 globin KI allele. | ||||
| Copyright | This image is from Huo Y, J Biol Chem 2009 Feb 20;284(8):4889-96 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:147906 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 10/29/2024 MGI 6.24 |
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