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| Caption | Generation of the Sv2btm1Baj allele. A) Targeting construct. A region of the Sv2b gene containing the first exon (depicted as a black box) was cloned from a mouse 129X1/SvJ genomic library. The genomic fragment was used to generate the indicated targeting vector in which exon 1 was replaced with a gene encoding neomycin resistance. A thymidine kinase gene, which makes cells sensitive to gancyclovir was placed at the end of the targeting construct to allow for negative selection of non-homologous recombination events. The mutant gene produces a smaller HindIII (H3) restriction fragment as detected in Southern analyses with a probe indicated by the band above the disrupted gene. Notation key: H2 = HindII restriction site, H3 = HindIII restriction site, RV = EcoRV restriction site, neo = DNA encoding neomycin resistance, TK = DNA encoding thymidine kinase. | ||||
| Copyright | This image is from Morgans CW, PLoS ONE 2009;4(4):e5230, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:148272 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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