Molecular Image Detail

Image

Caption

Targeting strategy for the Itgb1^tm5.1Ref (hpmKI^lox) allele. (A) Partial map of the Itgb1 (beta1A) wild-type (wt), the hpm knock-in allele before (hpmKI^neo-tk+) and after Cre-mediated deletion of the floxed neo-tk cassette (hpmKI^lox). External probes used for Southern blotting after BamHI digest and genotyping PCR primers are indicated. Cyto-cDNA, a wt 141 bp cDNA fragment coding for the complete cytoplasmic domain of the Itgb1 gene including the endogenous stop codon; E15cyto, endogenous sequence of exon 15 encoding for the membrane proximal part of the beta1 integrin cytoplasmic tail; filled boxes, exons; D, exon D; neo, neomycin resistance gene; tk, thymidine kinase gene; triangles, loxP sites; pA, polyadenylation signal.

This image is from Piwko-Czuchra A, PLoS ONE 2009;4(5):e5488, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:148885

Associated
Alleles

Symbol	Name
Itgb1^tm5.1Ref	integrin beta 1 (fibronectin receptor beta); targeted mutation 5.1, Reinhard Fassler

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