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| Caption | Generation of the Angptl3tm1Lex allele. (A) A schematic representation of the Angptl3 locus is shown, including relevant restriction sites used for confirmation Southern hybridization analysis (BglII and SpeI). A genomic clone was isolated (pKOS-78) containing the exon 1-4 region to be deleted. A yeast-mediated homologous recombination step first replaced the exon 1-4 region of the genomic clone with a yeast selection cassette. The final targeting vector was completed by replacing the yeast selection cassette with an ES selection cassette containing the LacZ reporter gene and a gene for G418 resistance. The 5' and 3' external probes used for Southern hybridization analysis were designed outside the target vector arms of homology and were generated by genomic PCR. | ||||||
| Copyright | This image is from Lee EC, J Biol Chem 2009 May 15;284(20):13735-45 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:149912 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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