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| Caption | Construction of the Angptl4 E40K point mutation gene targeting vector to generate the Angptl4tm2Lex allele. (A) A schematic representation of the Angptl4 locus is shown, including relevant restriction sites used for cloning and confirmation Southern hybridization analysis (BamHI, SfiI, BclI, and HindIII). The targeting vector was modified from the previously published Angptl4 conditional targeting vector. The new 5' arm of homology with the required mutation was amplified by PCR. This cDNA and the LoxP-neo-LoxP selection cassette were subcloned into the conditional targeting vector, replacing the old 5' arm of homology and selection cassette. The 5' internal and 3' external probes used for Southern hybridization analysis were designed inside and outside the target vector arms of homology accordingly and were generated by genomic PCR. | ||||
| Copyright | This image is from Lee EC, J Biol Chem 2009 May 15;284(20):13735-45 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:149912 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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