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Caption Construction of the Angptl4 E40K point mutation gene targeting vector to generate the Angptl4tm2Lex allele. (A) A schematic representation of the Angptl4 locus is shown, including relevant restriction sites used for cloning and confirmation Southern hybridization analysis (BamHI, SfiI, BclI, and HindIII). The targeting vector was modified from the previously published Angptl4 conditional targeting vector. The new 5' arm of homology with the required mutation was amplified by PCR. This cDNA and the LoxP-neo-LoxP selection cassette were subcloned into the conditional targeting vector, replacing the old 5' arm of homology and selection cassette. The 5' internal and 3' external probes used for Southern hybridization analysis were designed inside and outside the target vector arms of homology accordingly and were generated by genomic PCR.
Copyright This image is from Lee EC, J Biol Chem 2009 May 15;284(20):13735-45 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:149912
Associated
Alleles
Symbol Name
Angptl4tm2Lex angiopoietin-like 4; targeted mutation 2, Lexicon Pharmaceuticals

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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory