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| Caption | Generation of the Zfhx3tm1.1Jtd (Atbf1flox) allele in mice. (a) Design of cloning vectors and targeting strategies. Part of the Zfhx3 (Atbf1) gene (from exon 6 to exon 10) is shown at top (wildtype allele). LoxP and Frt sequences are marked by triangle and diamond-shaped boxes, respectively. The targeting construct was generated by inserting three Zfhx3 fragments (6.2, 0.9, and 4.7 kb) into pFlexible. Two hybridization probes (P1 and P2) used in Southern blot analyses are indicated in the mutant allele. The PGK-puro-delta-tk selection cassette was deleted by mating Atbf1PGK mice with ACT-FLPe mice to generate the Zfhx3tm1.1Jtd allele. Digestion of targeted genomic DNA by Xba I yield a 10.9 kb fragment for the wt allele and an 8.4 kb fragment for the mutant allele when the 5' probe (P1) is used, while digestion by Xho I yields 7.4 kb wt and 5.1 kb mutant bands when the 3' probe (P2) is used. | ||||
| Copyright | This image is from Sun X, Genesis 2012 Nov;50(11):819-27, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:189563 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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