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| Caption | Generation of the Myctm1.1(MYC)Gwbk allele. Targeting strategy for homologous recombination in ES cells. The endogenous murine Myc (c-Myc) gene was replaced with a humanized MYC gene (hc-Myc). hc-Myc has human DNA sequences from the first translation start side (CUG) up to 69 bp downstream of the stop codon (TAA). Non-coding sequences in exon 1 and exon 3 are of murine origin so that human MYC is expressed under the control of murine regulatory elements. (A) Endogenous murine Myc with its three exons (E1, E2, E3) and the three promoters (P1, P2, P3). Indicated are the two start codons (CUG, AUG) and the stop codon (TAA), as well as the flanking EcoRI restriction sites. (B) Murine Myc locus after homologous recombination with the targeting vector. Indicated in black are human sequences, in grey murine sequences and with triangles the two loxP-sites that flank the Neomycin-Geneticin resistance gene. (C) Recombined murine Myc locus after Cre-mediated deletion of the Neomycin Geneticin resistance gene. A piece of mouse chromosome 15 (position 61.985.920 to 61.989.995) is replaced by a piece of human chromosome 8 (position 128.748.840 to 128.753.273). Dotted lines indicate recombination events. This schematic view displays EcoRI restriction sites, length of fragments after EcoRI-digestion, the sequence in the junction region between human and murine elements and the regions where the Southern blot probes (5' probe and 3' probe) bind. Cre = Cre recombinase. | ||||
| Copyright | This image is from Lehmann FM, PLoS One 2012;7(7):e42021, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:189677 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/10/2024 MGI 6.24 |
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