Molecular Image Detail

Image

Caption

Generation of the Vsx2^tm1.1Itl and Vsx2^tm1.1Eml alleles. (B) Schematic representation of the targeting strategy. The R200Q and R227W mutations (asterisk) were introduced into exon 4 and the ACN cassette was cloned into intron 3 of Vsx2. These elements were then placed into the pTK1TK2 targeting vector. After homologous recombination, R200Q-ACN and R227W-ACN chimeric mice were generated. The ACN cassette was deleted by Cre recombination in the male germline generating the Vsx2^tm1.1Itl (R200Q) and Vsx2^tm1.1Eml (R227W) alleles. Chimeric males were crossed with wild-type females to generate germline transmitted R200Q and R227W heterozygous mice.

This image is from Zou C, PLoS Genet 2012 Sep;8(9):e1002924, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:190452

Associated
Alleles

Symbol	Name
Vsx2^tm1.1Eml	visual system homeobox 2; targeted mutation 1.1, Edward M Levine
Vsx2^tm1.1Itl	visual system homeobox 2; targeted mutation 1.1, inGenious Targeting Laboratory

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